Genome sequence of Leishmania mexicana MNYC/BZ/62/M379 expressing Cas9 and T7 RNA polymerase

نویسندگان

چکیده

We present the genome sequence of Leishmania mexicana MNYC/BZ/62/M379 modified to express Cas9 and T7 RNA-polymerase, revealing high similarity reference (MHOM/GT2001/U1103). Through RNAseq-based annotation coding sequences untranslated regions, we provide primer for construct sgRNA template generation CRISPR-assisted gene deletion endogenous tagging.

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ژورنال

عنوان ژورنال: Wellcome open research

سال: 2023

ISSN: ['2398-502X']

DOI: https://doi.org/10.12688/wellcomeopenres.18575.2